| Cloning
is a highly controversial and complicated issue both technically and "ethically".
I will attempt to explain some aspects regarding cloning in a rather simplified
form to make it somewhat easier for the non-specialized readers.
A
human cell contains
23
pairs of Chromosomes or 46 chromosomes
(somatic or body cells). Twenty three of them come from the
father through a sperm and 23 from the mother through an
ovum.
That's why only gametes (sperms and ova) have half the number of chromosomes
(23) that when unite together give a single cell with 46 chromosomes,
called a
Zygote or a fertilized ovum, that divide and divide
giving rise to an embryo that grows into a full mature human being from
one cell.
The
chromosomes are present in the nucleus of the cell and carry tens of
thousands of genes that are responsible for our external features, internal
body features, cellular function, susceptibility to certain diseases, or
even cause diseases itself, etc.... That's why genes are called units of
inheritance as every gene has a certain function that is transmitted from
a generation to another through reproduction.
With
the synthesis of sperms and ova in the testicles and ovaries, genes are
distributed in a random fashion. So, different sperms in the same individual
are NOT genetically identical 100% and the same thing is
present in the ova. Thus the genetic material of any individual (genes
or chromosomes), comes half from the father and half from the mother
in a RANDOM fashion depending on which sperm has fertilized which
ovum. Such genetic dissimilarities leads to the differences we observe
between brothers and sisters and the whole human race at large.
Such
biological variation is crucial for the continuity of the human race. If
human responses to environmental conditions and diseases are the same,
a single hazard, disease, or a microbe may be deadly to all of the human
race leading to its extinction. But the differences in responses of every
one of us to the same effector is protective to some and may be lethal
to others, maintaining the propagation of individuals at a familial level
and the human race at large.
Now
the next step is to discuss the miraculous divine power put in those genes
and how they control and master the growth of a single tiny cell, that
you can just blow away by a gentle blow of your mouth, to grow into a mature
individual who moves, sees, thinks, quarrels, and may be one day governs
the whole world.
I look
at it as a divine miraculous program that is present in the genes of sperms
and ova that, again in a miraculous way, know how to unite to form a 46
chromosomes
cell, how to move from the tube to reach its destination in the uterus
(womb), how to fix itself in its walls through fingers which is called
trophoblast, and how to get its blood supply from the mother to feed itself
and respire. It also then knows how to divide and divide into a multicellular
structure, then arrange itself in what we call an inner cell mass and an
outer cell mass that start to differentiate again in a miraculous way into
different types of tissues (skin, muscle, bone, blood, cartilage, nerves,
etc.), Additionally, every group of tissues know how to blend together
and to be organized to give highly specific organs like the eye, brain,
and heart, in the same place with the same function in every individual,
etc. This is something miraculous in itself. If you study embryology you
cannot but feel God's hands and you feel very small and trivial in front
of this divine power that is present in such a small "microscopic" piece
of DNA.
The
following illustrations show the travel of the Zygote through
the tube and womb and its implantation deep in the wall of the womb; the
second one shows division of the Zygote from a single cell into
a multicellular structure that will makeup the various tissues and organs
of the human embryo.

Such
a natural way of reproduction ensures selection of the best gametes available.
Weak immotile and malformed sperms are unable to travel all the way to
reach the ova and only the best healthy ovum reaches maturity in
the mid cycle while about
1000
other ova fail to reach maturity
every month in a woman's cycle. So, there is a process of natural selection
here that ensures that babies would be healthy and free of chromosomal
anomalies in more than 99% of cases (even malformed babies are recognized
by the womb and spontaneously expelled early in pregnancy via spontaneous
abortion). So, spontaneous abortion is not always something to regret as
it is in almost half of the cases a Godly way to ensure a healthy offspring.
The
next subject to be discussed is the forerunner of cloning, or what is called
in plain English, test tube babies as a mean of treating infertile couples.
Called
IVF & ET (In vitro* fertilization and embryo transfer),
It is a process in which Gynecologists:
| 1- Stimulate
the ovaries to produce many ova rather than one (superovulation).
2- Aspirate
the ova from the ovaries when they are mature enough.
3- Leave the
ova with the husband's** sperms in a test tube (actually in certain dishes)
in a certain media that resemble the fluid inside the woman's tubes.
4- Fertilization
of some of the ova (in vitro fertilization) occurs, followed by
their division to form early embryos.
5- Threeof
the embryos are transferred to the woman's womb when they are 2-3
days old (embryo transfer).
6- One or
more of the embryos will implant itself in the womb and pregnancy occurs. |
Only
three embryos are normally transferred, creating a problem with the rest
of the embryos.
What
to do with them?
Some
freeze them to be used in the next cycle if pregnancy did not occur and
others discard them.
There
are thousands of frozen embryos in IVF labs and doctors don't know
what to do with them. This is a substrate for cloning research as
we will see in a while, as the embryos are already there.
--------------------------------------------------------------
* In
Vitro
means outside the body.
**
in Western countries they may use donor sperms or eggs, something that
is not practiced in Egypt.
-------------------------------------------------------------
Scientists
became more familiar with the handling of the ova and sperms when they
succeeded in "forcing" fertilization in some cases in which in-vitro
fertilization failed to occur.
They
are now able to cut the tail of the sperm to get its head that contains
the 23 chromosomes and produce a small hole in the wall of the
ovum and inject the head of the sperm inside it effecting fertilization.
A process that is called ICSI or intra-cytoplasmic sperm injection.
In
the following 2 figures the process of ICSI can be seen in
a real lab (left) and in a diagrammatic representation (right)

These
advances paved the way to more and more research in the area of fertilization
and hence the era of cloning began.
Now
to address the issue of cloning.
We
have seen that both in natural conception and in IVF programs half
of the genetic material comes from the father and the other half comes
from the mother in a RANDOM manner. However, this is not the situation
in cloning.
Cloning
research aims at two different goals:
| 1- Reproductive
Cloning: To have a child through either embryo splitting or nuclear
transplantation using adult cells.
2-Therapeutic
Cloning: Cloning of embryos to obtain stem cells for research on organ
and tissue transplantation.
|
First: Embryo
splitting:
In
the very early stages of embryonic development, the cells of the embryo
are exactly the same and are pluripotent (a pluripotent cell is a cell
that is able to divide into all other types of cells and tissues).
This
mean that if you have a very early embryo (within the first 12 days
of embryonic life before cells become specialized) and you divided it into
4 or 8 groups of cells (an easy technology that is practiced
right now in animals cloning) it will grow into 4 or 8 "genetically
identical babies" (clones).
This
is already happening in nature with identical twin who were initially a
single embryo that divided very early into 2 embryos (and sometimes
three) before cellular differentiation or specialization.
The
question is what can this type of cloning serve in humans?
Obviously
nothing but scientific research, or to satisfy a strange request from a
couple to have an identical twin instead of a single baby, or perhaps one
day some parents will ask to have a copy of their baby frozen as a spare
in case something wrong happens to their child as some people believe that
one advantage of cloning is to compensate someone for the loss of a child
by cloning a replica of it.
Second: Nuclear
transplantation:

This
is the type of cloning Dr. Antinori wants to practice for his infertile
patients.
In
this type of cloning, there is no mother and father, there is no natural
selection and there is no individuality (unique "genetic" criteria
for every human being) as happens in natural conception.
Explanation follows:
In
this technique:
| 1- Embryologists
can get an egg from a female and an adult cell (a body cell that contain
46
chromosomes* not a sperm or egg) from the individual they want to clone.
2- They can
remove the nucleus of the egg (i.e. remove the 23 chromosomes* of
the egg).
3- They then
fuse the nucleus of the adult cell that contains 46 chromosomes* with
the de-nucleated egg (there are now different techniques to achieve this
fusion)
4-The chromosomes
of
the adult cell would be re-programmed by the egg cytoplasm and would
re-acquire the memory of division and starts dividing to produce an embryo.
5- The resulting
embryo can be transferred, then, to the womb of another female (or the
same female from whom they got the ovum or adult cell) to allow
for the completion of pregnancy. |
--------------------------------------
*
I used here the number of chromosomes in humans to simplify understanding
the procedure.
--------------------------------------
By
this method, the clone (the baby) will be genetically identical to the
owner of the adult cell, as all of its genetic material has been gained
from this cell only, while the woman acts here as a receptacle just for
the growth of the baby. She is not a genetic mother. She is a surrogate
mother unless the adult cell itself was hers.
These
techniques pose many hazards:
1- Dolly,
the famous first mammalian clone, cost its cloner 277 embryos/baby
sheep to have a successful clone. Other embryos either aborted, died, or
were born malformed. But the announcements in the media was only for the
one successful trial masking the miserable 276 failed, dead, or
malformed embryos/sheep.
2- Dr. Wilmut,
who cloned Dolly, is against human cloning himself because he knows
the problems that he faced with the other cloned embryos/sheep.

3- Dolly itself
has a cellular age that is 6 years older than its actual age raising
the possibility of premature aging of the clone.
4- The other,
more recent technique, that was used to clone mice is only successful in
less than 3% of the attempts, and also yields dead or malformed
clones in most of the attempts.
5- The long
term consequences of cloning on the cloned animals is not yet known or
enough studied. Many of the cloned animals suffered diseases and some suffered
morbid obesity.
6- Our understanding
of cloning and how cells grow normally (or abnormally) is not yet complete
enough to risk it in humans.
7- The effect
of playing with the human genome at large is not yet fully understood and
may get out of control, or yield a new trait that may threaten the whole
human race.
8- Perhaps
some lunatic scientists may use cloning to produce a clone of people to
be used for a certain purpose or trying to improve the human race.
The above are only
some of the aspects that make many doctors refuse cloning on a scientific
base rather than on an ethical or a religious base (that of course has
a lot against reproductive cloning) |
Third: Therapeutic
cloning:
This
type of cloning aims at creating embryos to get their pluripotent cells
(the so called
Embryonic Stem Cells) to experiment with. The rest
of the embryo is then discarded. Actually embryologists make use
of the frozen embryos in IVF labs after getting the consent of the
parents to use their "extra" embryos for scientific research. As
I said, the pool is already there.
Researchers
want to study the possibility of stimulating stem cells using different
media and/or techniques to "direct" its development into certain
planned direction to produce a specific tissue or organ.
The
aim of using this technique, if successful, would be as follows:
Scientists
believe that they can treat certain diseases that are untreatable now with
the use of stem cell cloning.
Suppose
that someone develops a certain disease such as Alzheimer, Parkinsonism,
kidney
failure, liver failure, or diabetes. They think that
the scenario can go as follows:
| 1- An adult
cell can be taken from the patient's OWN body
2- An embryo
could be created as described in the nuclear transplantation method above
using this adult cell with the help of an egg from any woman.
3- Stem cells
are then collected from this embryo in its very early stages of development.
4- Stem cells
can be used to be transformed into nerve cells that can be used to treat
Alzheimer or Parkinsonism, into a kidney or a liver
that
can be used for transplantation, or into pancreatic tissue that
can be used to treat diabetes instead of the damaged pancreas |
The
idea here is to get a tissue or an organ that is immunologically identical
to the person so it won't be rejected.
Stem
cell research is currently in process, but is in its very early stages.
However, the question is why don't geneticists and embryologists experiment,
as they wish, with animal stem cells first and keep away from human stem
cells and human embryos till they know for sure the whole story in animal
research before they "play" with human embryos?
Finally,
cloning literally means "to have a copy of something." Here it is
meant to be a genetic copy of another individual. So, the first thing that
can be criticized in cloning is that someone will give him/herself the
right to decide the genetic makeup and traits (properties) of another human
being. Someone can ask for a baby who is genetically identical to himself
(to satisfy his self ego), or identical to someone else whom he thinks
has good traits. It won't cost him/her more than a single cell, but will
cost the cloned individual the chance to be a unique individual that has
its own genetic makeup that for sure will be reflected on his behavior
and whole career.
NB: This was
meant to be a simplified, but scientific, explanation of the subject. I
will not go into the many ethical and religious constraints on cloning
as it differs between societies and individuals according to their background,
set of ethics, values, and of course religious beliefs.

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